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mtmDB is a transposon library in maize for the purpose of targeted selection of gene knockouts. It currently contains 43,776 DNA samples from F1 insertion plants and 43,776 F2 progeny which have been generated and developed by the collaboration of Rob Martienssen (Cold Spring Harbor Laboratory), Michael Freeling (Berkeley) and Danny Alexander (Syngenta). It is a public repository of maize mutant information including pedigree, phenotype, strain, and DNA sequence. All strains are gridded and barcoded, and can be ordered electronically via this site or from the Maize Co-operative Stock center. For details see May et al. (2003). Maize-targeted mutagenesis: A knockout resource for maize. May BP, Liu H, Vollbrecht E, Senior L, Rabinowicz PD, Roh D, Pan X, Stein L, Freeling M, Alexander D, Martienssen R. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11541-6. Epub 2003 Sep 3. The database provides several search options such as object oriented search (Simple Search, Class Search), Text Search and search with ACEDB queries. To inspect gene knockouts,Ê select "simple search" "gene" andÊ enter "**" into the search field.Ê Allow a few moments for the list to appear. We are temporarily unable to take further requests for custom gene knockouts, pending further funding, but we hope to provide gene knockout information via next-generation sequencing in the near future. Please contact Michael Regulski or Jong-Jin Hong for mutant related information.Maintained by Matthew Vaughn |
